<p>Researcher from Hokkaido University recently clears clue over DNA replication and declares the concepts how DNA replication always moves towards forward direction.</p><p>&amp;quot;When DNA is replicated, one of the two chains can be copied, or synthesized, in a continuous manner while the other chain is synthesized in many fragments that need to be joined later,&amp;quot; says Min Yao from Hokkaido University. &amp;quot;One of the big questions in biology has been why cells don&#039;t have a reverse-direction enzyme so that both chains can be synthesized efficiently.&amp;quot;</p><p>Researcher found that group of enzyme named Thg1-like proteins (TLPs), which were found to add nucleotides in the opposite direction. TLPs are the exception and add nucleotides in the reverse direction to repair the &amp;quot;opposite end&amp;quot; of damaged RNAs.<br /><br /><a href="http://www.srlchem.com/" target="_blank" rel="noopener noreferrer"><img src="http://www.microbiozindia.com/images/ASX.png" alt="" /></a></p><p>Researcher used X-ray Crystallography to analyse structure of TLPs/RNA this sturdy gave researcher into the complex mechanism that TLPs employ to add nucleotides in the reverse direction. &amp;quot;By comparing the molecular mechanisms of forward and reverse reactions in more detail, we would like to fully understand the evolutionary context of DNA replication,&amp;quot; says Yao.</p><p><strong>Story source and Credit :</strong> Hokkaido University</p><p>Journal References</p><p>S. Kimura, T. Suzuki, M. Chen, K. Kato, J. Yu, A. Nakamura, I. Tanaka, M. Yao. Template-dependent nucleotide addition in the reverse (3&#039;-5&#039;) direction by Thg1-like protein. Science Advances, 2016; 2 (3): e1501397 DOI: 10.1126/sciadv.1501397</p>